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Chapters
- 00:05Title
- 00:48Embryo Collection for Time-lapse Imaging
- 01:51Embryo Preparation
- 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
- 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
- 07:26Post-acquisition Processing
- 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
- 09:29Conclusion
En kombination af de avancerede optiske teknikker i laser scanning mikroskopi med lang bølgelængde multi photon fluorescens excitation blev implementeret for at fange høj opløsning, tre-dimensionelle, real-time imaging af neural crest migration i Tg (sox10:EGFP) og Tg (foxd3:GFP) zebrafisk embryoner.
