Journal
/
/
サンプル準備およびゼブラフィッシュから RNASeq による遺伝子発現データの解析
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
DOI:

11:42 min

October 27, 2017

, ,

Chapters

  • 00:05Title
  • 01:04Generating Embryos Through Natural Mating and Staging Embryos
  • 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
  • 04:55Sorting Differentially Expressed Genes
  • 07:00Determine Enriched Pathways
  • 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
  • 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
  • 10:47Conclusion

Summary

자동 번역

このプロトコルは、ゼブラフィッシュの胚は、幼虫から全体のトランスクリプトーム解析手法を提案またはセルを並べ替えられます。我々 には、RNA の隔離、RNASeq データの経路解析と遺伝子発現変動の qRT PCR による検証。

Related Videos

Read Article