È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
10,491 Views
•
11:42 min
•
October 27, 2017
DOI:
Summary
Automatically generated
This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.
Read Article
Cite this Article
Hostelley, T. L., Nesmith, J. E., Zaghloul, N. A. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (128), e56187, doi:10.3791/56187 (2017).
Copy