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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
JoVE Journal
Genetica
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JoVE Journal Genetica
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

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11:42 min

October 27, 2017

DOI:

11:42 min
October 27, 2017

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Summary

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This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.

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