Journal
/
발생학
/
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

6,587 Views

•

12:30 min

•

October 06, 2017

DOI:

10.3791/56121-v

DOI:
10.3791/56121-v

•

12:30 min
October 06, 2017

•

2 Views
, , , ,
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health,Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children’s Hospital

Summary

Automatically generated

We report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. A detailed procedure for the construction, packaging, and quantitative analysis of the rAaeDV vectors as well as for larval infection is described.

Explore More Videos

Recombinant Mosquito DensovirusGene Delivery VectorFunctional AnalysisMosquito LarvaeViral-based DeliveryTransduction EfficiencyPersistent EffectsMiRNAShRNAAedes AegyptiGene FunctionGene OverexpressionGene Knockdown

Read Article

Cite this Article

Liu, P., Xu, J., Dong, Y., Chen, X., Gu, J. Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae. J. Vis. Exp. (128), e56121, doi:10.3791/56121 (2017).

Download .ris file

Copy

Share Video

.

Copy