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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

DOI:
10.3791/56091-v

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11:27 min

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November 02, 2018

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, , ,
1Department of Chemistry & Biochemistry,University of California, San Diego, 2Department of Biophysics,Bose Institute

Chapters

  • 00:05Title
  • 01:40Preparation of Recombinant Virus Suitable for Large Scale Expression of IKK1/α
  • 03:41Purification of His-IKK1
  • 05:54Screen for Crystallization Condition of IKK1
  • 06:47Growing Crystals in Large Numbers
  • 07:45Cryo Protection of Crystals
  • 08:57Results: Crystallization, and Structure Determination of Human IKK1/α
  • 10:34Conclusion

Summary

자동 번역

자동 번역

IκB Kinase 1/α (IKK1/α CHUK) is a Ser/Thr protein kinase that is involved in a myriad of cellular activities primarily through activation of NF-κB transcription factors. Here, we describe the main steps necessary for the production and crystal structure determination of this protein.

Tags

IKK1-alphaHumanProtein ExpressionInsect CellsSf9 CellsTransfectionCrystallizationStructure DeterminationMolecular Replacement

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