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08:24 min
August 18, 2017
DOI:
10.3791/56037-v
在这里我们目前用于洗涤剂免费培养的哺乳动物细胞基于氮空和随后的分离,胞浆和膜结合蛋白的超速离心同质化的协议。此方法是理想的监测分区之间可溶性的外周膜蛋白和膜组分。
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Zhou, M., Philips, M. R. Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells. J. Vis. Exp. (126), e56037, doi:10.3791/56037 (2017).
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