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Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
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JoVE Journal Bioquímica
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

15,392 Views

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08:24 min

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August 18, 2017

DOI:

10.3791/56037-v

DOI:
10.3791/56037-v

•

08:24 min
August 18, 2017

•

13 Views
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1Langone Medical Center,New York University

Tags

Nitrogen CavitationDifferential CentrifugationPeripheral Membrane ProteinsCultured CellsSubcellular CompartmentalizationHEK293 CellsHomogenization BufferPressurizationProtease InhibitorCell DisruptionFractionationSoluble FractionMembrane Fraction

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Zhou, M., Philips, M. R. Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells. J. Vis. Exp. (126), e56037, doi:10.3791/56037 (2017).

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