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Chapters
- 00:05Title
- 01:08Preparation of an ECS Machine
- 01:47Induction of Acute ECS
- 03:07Homogenization and Fractionation of Rat Hippocampi
- 05:20Isolation of the PSD from the Crude Membrane Protein Fraction
- 06:32Results: Examination of Synaptic Proteins in Hippocampal S2, P2, and PSD Fractions from Rats that Received NS or Acute ECS
- 07:55Conclusion
Electroconvulsive seizure (ECS) is an experimental animal model of electroconvulsive therapy for severe depression. ECS globally stimulates activity in the hippocampus, leading to synaptogenesis and synaptic plasticity. Here, we describe methods for ECS induction in rats and for subcellular fractionation of their hippocampi to examine seizure-induced changes in synaptic proteins.
