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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

10,583 Views

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09:32 min

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September 10, 2017

DOI:

10.3791/55911-v

DOI:
10.3791/55911-v

•

09:32 min
September 10, 2017

•

3 Views
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1National Health and Environmental Effects Research Laboratory,U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

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A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.

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Capillary ElectrophoresisImmunoassayAutomated ProcedureProtein QuantitationCell CultureBiofluidsWestern BlotSample PreparationMaster MixDenaturing ConditionsAntibody Dilutions

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Nelson, G. M., Guynn, J. M., Chorley, B. N. Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method. J. Vis. Exp. (127), e55911, doi:10.3791/55911 (2017).

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