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הבחירה תלויה עצמאית הדור של CRISPR / Cas9 בתיווך גין Knockouts בתאי יונקים
Chapters
- 00:05Title
- 00:57Transfection of CRISPER Component into Cultured Cells and Drug Selection
- 02:31Isolation of Clonal Populations
- 03:53Screening Candidates by Dot Blot
- 06:30Screening Candidates by Colony PCR
- 08:59Results: Knockout Cell Lines Generated by Two CRISPER/Cas9 Methods
- 10:40Conclusion
