Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
12,174 Views
•
11:35 min
•
June 16, 2017
DOI:
Özet
Automatically generated
Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.
Read Article
Cite this Article
Sternburg, E. L., Dias, K. C., Karginov, F. V. Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. J. Vis. Exp. (124), e55903, doi:10.3791/55903 (2017).
Copy