This content is Free Access.
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
1Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics,The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics,The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science,Wenzhou Medical University, 5School of Pharmaceutical Science,Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre,Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology,Wenzhou Medical University
Chapters
- 00:00Title
- 01:15Follicle Isolation and Encapsulation
- 04:29In Vitro Culture of Encapsulated Follicles Under Simulated Microgravity Treatment
- 08:28Changing Culture Medium
- 09:17Follicle Retrieval
- 09:49Results
- 10:24Conclusion
A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
