A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
Zhang, S., Wu, Y., Weng, Y., Xu, Z., Chen, W., Zheng, D., Lin, W., Liu, J., Zhou, Y. In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity. J. Vis. Exp. (130), e55641, doi:10.3791/55641 (2017).