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Surgical Ablation Assay for Studying Eye Regeneration in Planarians
Chapters
- 00:05Title
- 00:50Animal Culture and Handling
- 01:25Surgery Surface Preparation
- 03:02Surgical Ablation
- 05:50Results: Planarian Eye Regeneration After Surgical Ablation
- 06:50Conclusion
This protocol shows how to consistently excise planarian eyes (optic cups) without disturbing surrounding tissues. Using an insulin needle and syringe, either one or both eyes can be ablated to facilitate investigations into the mechanisms regulating eye regeneration, the evolution of visual regeneration, and the neural basis of light-induced behavior.
