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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

9,853 Views

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08:03 min

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March 28, 2017

DOI:

10.3791/55558-v

DOI:
10.3791/55558-v

•

08:03 min
March 28, 2017

•

3 Views
, , ,
1Department of Biology,Georgetown University, 2Division of Infectious Diseases,Weill Cornell Medical College, 3Department of Medicine,Weill Cornell Medical College

Summary

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Here we describe a protocol for the extraction of metabolites from Staphylococcus aureus and their subsequent analysis via liquid chromatography and mass spectrometry.

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Tandem Liquid Chromatography-Mass SpectrometryMetabolite AnalysisStaphylococcus AureusMetabolismPathogenesisNutrient DepletionHost InfectionOptical DensityExtraction BufferQuenching Solution

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Samuels, D. J., Wang, Z., Rhee, K. Y., Brinsmade, S. R. A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus. J. Vis. Exp. (121), e55558, doi:10.3791/55558 (2017).

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