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Biochemistry

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JoVE Journal Biochemistry
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
 

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Article DOI: 10.3791/55482-v • 06:34 min • March 27th, 2017
March 27th, 2017 •
1, 1, 1, 1
1Department of Chemistry, University of California, Santa Cruz

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Herein we report a tailored HPLC purification protocol that yields high-purity amyloid beta 42 (Aβ42) and amyloid beta 40 (Aβ40) peptides, capable of oligomer formation. Amyloid beta is a highly aggregation prone, hydrophobic peptide implicated in Alzheimer's disease. The amyloidogenic nature of the peptide makes its purification a challenge.

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HPLC Purification High-purity Amyloid Beta 42 Amyloid Beta 40 Peptides Oligomer Formation Biochemical Properties Challenging HPLC Protocol Buffers A And B Sample Dissolution Pump Inlets Polymer Tubing One Piece Fittings Preparative Column Dual Wavelength Detector HPLC Setup Software Wavelength Detector Nanometers Output Valve Sample Collection Tubing HPLC Software
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