The low-energy water-accommodated fraction (LEWAF) of crude oil is a challenging system to analyze, because over time, this complex mixture undergoes chemical transformations. This protocol illustrates methods for the preparation of the LEWAF sample and for performing photo-irradiation and chemical analysis by trapped ion mobility spectrometry–FT-ICR MS.
Benigni, P., Marin, R., Sandoval, K., Gardinali, P., Fernandez-Lima, F. Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS. J. Vis. Exp. (121), e55352, doi:10.3791/55352 (2017).