Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.
Natsume, Y., Wen, H., Zhu, T., Itoh, K., Sheng, L., Kurihara, K. Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion. J. Vis. Exp. (119), e55282, doi:10.3791/55282 (2017).