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Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
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Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
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January 24, 2017
DOI:
1Department of Mathematical and Physical Sciences, Faculty of Science,Japan Women’s University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience,National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science,National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology,The University of Tokyo
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Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.
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Natsume, Y., Wen, H., Zhu, T., Itoh, K., Sheng, L., Kurihara, K. Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion. J. Vis. Exp. (119), e55282, doi:10.3791/55282 (2017).
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