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Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
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Chapters
- 00:05Title
- 01:13Chicken Ovalbumin-specific TH1 (cOVA-TH1) Cell isolation and Antigen Presenting Cell Co-culture
- 04:42cOVA-TH1 Cell Radiolabeling and Uptake and Efflux Analysis
- 06:44Positron Emission Tomograph–Computed Tomography (PET/CT) In Vivo Imaging
- 08:26Results: Representative cOVA-TH1 Cell Tracking In Vitro and In Vivo
- 10:32Conclusion
Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).
