JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
Chapters
- 00:05Title
- 01:213T3 Feeder Cell Culture and Irradiation
- 02:31Pediatric Esophageal Biospy Acquisition and Processing
- 05:00Cell Culture Expansion, Freezing, and Storage
- 07:17Results: Representative Human Esophageal Cell Characterization
- 08:59Conclusion
Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.
