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Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
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Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

6,859 Views

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10:15 min

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March 22, 2017

DOI:

10.3791/55243-v

DOI:
10.3791/55243-v

•

10:15 min
March 22, 2017

•

8 Views
, , ,
1Department of Pediatrics,UConn Health, 2Department of Gastroenterology,Connecticut Children’s Medical Center, 3Department of Surgery,Connecticut Children’s Medical Center

Summary

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Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.

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Conditional ReprogrammingEsophageal Epithelial CellsDisease InvestigationEosinophilic EsophagitisEsophageal Atresia3D ScaffoldPatient-specificEndoscopic BiopsyFeeder Cells3T3 CellsIrradiationConditional Programming MediumSerum-free Keratinocyte MediumPrimocinFormalinCryomold

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Cite this Article

Jensen, T. J., Foster, C., Sayej, W., Finck, C. M. Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation. J. Vis. Exp. (121), e55243, doi:10.3791/55243 (2017).

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