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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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May 11, 2017
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1Department of Health, Animal Science and Food Safety,University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE,Université de Tours, INRA, 4PAO,INRA, 5Clinique des Animaux de Compagnie et des Équidés,Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania
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This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.
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Franciosi, F., Tessaro, I., Dalbies-Tran, R., Douet, C., Reigner, F., Deleuze, S., Papillier, P., Miclea, I., Lodde, V., Luciano, A. M., Goudet, G. Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes. J. Vis. Exp. (123), e55242, doi:10.3791/55242 (2017).
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