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从人类脐带和胎盘胎儿分离和鉴定间充质干细胞
JoVE 신문
발생학
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JoVE 신문 발생학
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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从人类脐带和胎盘胎儿分离和鉴定间充质干细胞

DOI:
10.3791/55224-v

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07:06 min

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April 03, 2017

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, , , , , ,
1Department of Biological Sciences,Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology,St. John Provindence – Providence Park Hospital, 4Department of Neurosurgery,Beaumont Health System

Chapters

  • 00:05Title
  • 01:09Human Umbilical Cord (UC), Cord-Placenta Junction (CPJ), and Fetal Placenta (FP) Processing and Cell Isolation
  • 04:29Results: Colony-Forming Efficiency and Mesenchmyal Stromal Cell (MSC) Marker Expression of Perinatal Tissue-Isolated Cells
  • 06:21Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一个协议，用于人脐带（UC）和胎儿胎盘样品引入帘线衬（CL），脐带胶质（WJ），索胎盘结（CPJ），和胎儿胎盘（FP）为隔离的夹层以及使用该外植体培养技术间充质基质细胞（MSC）的表征。

Tags

Keywords: Mesenchymal Stromal CellsUmbilical CordFetal PlacentaStem Cell BiologyAlternative MedicineMulti-potentCord Mesenchymal Stromal CellsAnatomical RegionsUmbilical CordWharton’s JellyEpitheliumSubamnionBlood VesselsPerivascular JellyTrypsinPartial Digestion

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