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Visualisierung von Protein-Protein-Wechselwirkung in Kern- und Cytoplasma-Fraktionen durch Co-Immunpräzipitation und<em> In Situ</em> Nähe Ligatur Assay
Chapters
- 00:05Title
- 01:01Co-immunoprecipitation
- 04:14Immunocytochemistry
- 06:15Proximity Ligation Assay
- 08:19Results: Visualization of NF90-RBM3 Interactions in Nuclear and Cytoplasmic Fractions of Cells
- 09:14Conclusion
Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.
