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QTL定位和CRISPR / Cas9编辑识别抗药性基因<em>弓形虫</em
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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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QTL定位和CRISPR / Cas9编辑识别抗药性基因<em>弓形虫</em

15,905 Views

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11:37 min

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June 22, 2017

DOI:

10.3791/55185-v

DOI:
10.3791/55185-v

•

11:37 min
June 22, 2017

•

9 Views
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1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine,Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine,Louisiana State University

Summary

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详细介绍了如何使用基于全基因组序列的遗传图谱进行QTL定位，以鉴定弓形虫中的药物抗性基因，以及如何使用有效编辑基因组靶标的CRISPR / Cas9系统进行验证，在这种情况下耐药基因。

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Cite this Article

Shen, B., Powell, R. H., Behnke, M. S. QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii. J. Vis. Exp. (124), e55185, doi:10.3791/55185 (2017).

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