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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
JoVE Journal
Genetica
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JoVE Journal Genetica
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

15,918 Views

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11:37 min

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June 22, 2017

DOI:

10.3791/55185-v

DOI:
10.3791/55185-v

•

11:37 min
June 22, 2017

•

9 Views
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1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine,Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine,Louisiana State University

Summary

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Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.

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QTL MappingCRISPR/Cas9Drug ResistanceToxoplasma GondiiSinefunginWhole Genome SequencingSNP AnalysisGenetic ManipulationParasite Growth AssayQuantitative Trait LocusGenetic BasisPathogenesis

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Cite this Article

Shen, B., Powell, R. H., Behnke, M. S. QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii. J. Vis. Exp. (124), e55185, doi:10.3791/55185 (2017).

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