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Chapters
- 00:05Title
- 01:16Harvesting and Isolation of ASCs
- 03:19FACS Sorting for BMPR-1B Positive Cells
- 05:39Creation of Calvarial Defects and Application of ACS-containing Scaffold
- 07:03Results: Bone Regeneration in Response to BMPR-1B-positive ASC
- 08:02Conclusion
Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.
