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开源高内涵分析利用自动荧光寿命成像显微镜
 
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开源高内涵分析利用自动荧光寿命成像显微镜

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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我们目前使用的测定使用荧光共振能量转移（FRET）的读数蛋白质相互作用自动荧光寿命成像（FLIM）一个开源的高内涵分析（HCA）的仪器。此openFLIM-HCA仪数据采集是通过软件写入μManager和数据分析在FLIMfit进行控制。

Tags

生物物理学，第119，荧光显微镜，FLIM，HCA，开源，FRET，自动显微镜，药物发现
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