This video article details a straightforward in vivo methodology that can be used to systematically and efficiently characterize components of complex signaling pathways and regulatory networks in many invertebrate embryos.
Range, R. C., Martinez-Bartolomé, M., Burr, S. D. The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions. J. Vis. Exp. (120), e55113, doi:10.3791/55113 (2017).