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成像中性粒细胞吞噬体和细胞质使用比例测量pH指示剂
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면역학 및 감염병학
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Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
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成像中性粒细胞吞噬体和细胞质使用比例测量pH指示剂

9,815 Views

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12:42 min

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April 05, 2017

DOI:

10.3791/55107-v

DOI:
10.3791/55107-v

•

12:42 min
April 05, 2017

•

10 Views
, , , ,
1Centre for Molecular Medicine, Division of Medicine,University College London, 2Cell and Developmental Biology,University College London

Summary

Automatically generated

这手稿描述了一种简单的方法来测量吞噬体的pH值和面积以及使用比率量度指示器seminaphthorhodafluor（SNARF）-1，或S-1人和小鼠嗜中性粒细胞的胞质pH值。这是使用活细胞共聚焦荧光显微镜和图像分析来实现的。

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Foote, J. R., Levine, A. P., Behe, P., Duchen, M. R., Segal, A. W. Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator. J. Vis. Exp. (122), e55107, doi:10.3791/55107 (2017).

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