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Dextran effektiviserer Lentiviral transduktion af Murine og menneskelige primære NK-celler
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1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Chapters
- 00:05Title
- 00:44Purification and Expansion of Murine Primary NK Cells
- 02:03Purification and Expansion of Human Primary NK Cells
- 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
- 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
- 07:58Conclusion
Målet med denne undersøgelse var at formulere teknologier, der muliggør vellykket gen transduktion i primære natural killer (NK) celler. Dextran-medieret lentiviral transduktion af menneskelige eller musen primære NK celler resultater i højere gen expression effektivitet. Denne metode af genet transduktion vil langt forbedre NK celler genetisk manipulation.
