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Dextrane augmente l’efficacité de la Transduction des gènes des cellules NK primaires murines et humaines
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1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Chapters
- 00:05Title
- 00:44Purification and Expansion of Murine Primary NK Cells
- 02:03Purification and Expansion of Human Primary NK Cells
- 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
- 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
- 07:58Conclusion
L’objectif de cette étude était de formuler des technologies permettant la transduction réussie de gène dans les cellules primaires de le natural killer (NK). L’induite par le dextran de transduction des gènes de l’homme ou les résultats de cellules NK primaires de souris à une efficacité accrue les expression de gène. Cette méthode de transduction gène améliorera la manipulation génétique de cellules NK.
