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मूल्यांकन करने के लिए एक Monocyte Monolayer परख का उपयोग Fcγ रिसेप्टर की मध्यस्थता Phagocytosis
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
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मूल्यांकन करने के लिए एक Monocyte Monolayer परख का उपयोग Fcγ रिसेप्टर की मध्यस्थता Phagocytosis

17,307 Views

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06:27 min

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January 02, 2017

DOI:

10.3791/55039-v

DOI:
10.3791/55039-v

•

06:27 min
January 02, 2017

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4 Views
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1Department of Laboratory Medicine and Pathobiology,University of Toronto, 2Centre for Innovation,Canadian Blood Services

Tags

Monocyte Monolayer AssayFc Receptor-mediated PhagocytosisImmunohematologyBlood TransfusionAuto-antibodiesAllo-antibodiesRed Blood CellsOpsonizationPolyclonal Anti-D AntibodiesPBMCDensity Gradient CentrifugationIn Vitro Functional Assay

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Cite this Article

Tong, T. N., Branch, D. R. Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis. J. Vis. Exp. (119), e55039, doi:10.3791/55039 (2017).

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