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Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

17,540 Views

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06:27 min

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January 02, 2017

DOI:

10.3791/55039-v

DOI:
10.3791/55039-v

•

06:27 min
January 02, 2017

•

26 Views
,
1Department of Laboratory Medicine and Pathobiology,University of Toronto, 2Centre for Innovation,Canadian Blood Services

Summary

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The monocyte monolayer assay (MMA) is an in vitro assay that utilizes isolated primary monocytes obtained from mammalian peripheral whole blood to evaluate Fcγ receptor (FcγR)-mediated phagocytosis.

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Cite this Article

Tong, T. N., Branch, D. R. Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis. J. Vis. Exp. (119), e55039, doi:10.3791/55039 (2017).

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