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Fluss Virometry zur Analyse Antigenic Spektren von Virionen und extracellular Vesikeln
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Fluss Virometry zur Analyse Antigenic Spektren von Virionen und extracellular Vesikeln

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06:28 min

January 25, 2017

DOI:

06:28 min
January 25, 2017

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Summary

Automatically generated

Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

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