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Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

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06:28 min

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January 25, 2017

DOI:

10.3791/55020-v

DOI:
10.3791/55020-v

•

06:28 min
January 25, 2017

•

6 Views
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1Section on Intercellular Interactions,Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department,Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center

Summary

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Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

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Flow VirometryAntigenic SpectraVirionsExtracellular VesiclesMagnetic ParticlesMNPsFlow CytometryViral ParticlesAntibodiesCaptureDetectionMagnetic Separation

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Arakelyan, A., Fitzgerald, W., Zicari, S., Vagida, M., Grivel, J., Margolis, L. Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles. J. Vis. Exp. (119), e55020, doi:10.3791/55020 (2017).

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