01:05T-cell Receptor (TCR) and Human Leukocyte Antigen-resticted Tumor-derived Peptide (pHLA) Refolding and Purification by Fast Protein Liquid Chromatography (FPLC)
03:43Surface Plasmon Resonance (SPR) Equilibrium Binding and Thermodynamic Analyses
05:55Protein Crystallization and X-ray Diffraction
07:39Results: Molecular Analysis of gp100280-288 Recognition by CD8+ T-cells
Here, we describe methods that we commonly employ in the laboratory to determine how the nature of the interaction between the T-cell receptor and tumor antigens, presented by human leukocyte antigens, governs T-cell functionality; these methods include protein production, X-ray crystallography, biophysics, and functional T-cell experiments.