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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

DOI:
10.3791/54971-v

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08:53 min

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January 11, 2017

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,
1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Chapters

  • 00:05Title
  • 00:58Scaffold Liposome Preparation
  • 02:49Use of Scaffold Liposomes for Protein Binding Analysis
  • 04:25Use of Scaffold Liposomes for Enzymatic Assay
  • 06:08Results: Reconstitution of Lipid-proximal Protein-protein Interactions via Scaffold Liposomes
  • 08:06Conclusion

Summary

자동 번역

자동 번역

This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

Tags

Scaffold LiposomesLipid-proximal Protein-protein InteractionsMitochondrial DynamicsProtein ComplexesMembrane CompartmentsLipid TemplatesNative ConfirmationLipid CofactorsMitochondrial Fission MachineryHis-tagged ProteinsMffBuffer ABMELipid Extrusion

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