/

/

在线体积排阻和离子交换色谱法SAXS光束线

JoVE 신문
생화학

This content is Free Access.

JoVE 신문 생화학

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Please note that all translations are automatically generated. Click here for the English version.

在线体积排阻和离子交换色谱法SAXS光束线

16,895 Views

•

11:09 min

•

January 05, 2017

DOI:

10.3791/54861-v

DOI:

10.3791/54861-v

•

11:09 min

January 05, 2017

•

5 Views

Martha E. Brennich¹, Adam R. Round^2,3, Stephanie Hutin⁴

¹Structural Biology Group,European Synchrotron Radiation Facility, ²European Molecular Biology Laboratory, ³School of Chemical and Physical Sciences,Keele University, ⁴Groupe de Microscopie Electronique et Méthodes,Institut de Biologie Structurale

Summary

通过小角X射线散射（SAXS）的蛋白的溶液结构的确定需要单分散样品。在这里，我们提出了两种可能性，以确保样品制备和数据采集之间的最小延迟：在线尺寸排阻色谱（SEC）和在线离子交换层析（IEC）。

Explore More Videos

Online Size-exclusion Chromatography Ion-exchange Chromatography SAXS Beamline Structural Biology SAXS Molar Dispersed Samples SEC Gel Filtration Buffer HPLC System SAXS Flow-through Capillary Radiation Damage Protein Sample Aggregates HPLC Compatible Glass Vial UV Vis Data

Read Article