Journal
/
유전학
/
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
JoVE 신문
유전학
This content is Free Access.
JoVE 신문 유전학
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

9,461 Views

•

10:51 min

•

December 05, 2016

DOI:

10.3791/54831-v

DOI:
10.3791/54831-v

•

10:51 min
December 05, 2016

•

3 Views
, , ,
1Laboratory of Cell Cycle Genetics,The Rockefeller University, 2Sainsbury Laboratory,University of Cambridge

Summary

Automatically generated

Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.

Explore More Videos

Chlamydomonas ReinhardtiiTemperature-sensitive Lethal MutantsHigh-throughput RoboticsUV MutagenesisEssential GenesPathwaysCell CycleLiquid DispenserPlateUV Lamp

Read Article

Cite this Article

Breker, M., Lieberman, K., Tulin, F., Cross, F. R. High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii. J. Vis. Exp. (118), e54831, doi:10.3791/54831 (2016).

Download .ris file

Copy

Share Video

.

Copy