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Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
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Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

12,146 Views

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08:14 min

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March 17, 2017

DOI:

10.3791/54779-v

DOI:
10.3791/54779-v

•

08:14 min
March 17, 2017

•

1 Views
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1Center for Excellence in Post-Harvest Technologies,North Carolina A&T State University, North Carolina Research Campus, 2Human Performance Laboratory,Appalachian State University, North Carolina Research Campus

Summary

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This work describes an advanced workflow for the accurate and fast determination of NK (Natural Killer) cell count and NK cell cytotoxicity in human blood samples.

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Flow CytometryNatural Killer (NK) CellsCytotoxicityWhole BloodCell SeparationK562 CellsPropidium IodideImmunology

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Cite this Article

McBride, J. E., Meaney, M. P., John, C., Nieman, D. C., Warin, R. F. Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood. J. Vis. Exp. (121), e54779, doi:10.3791/54779 (2017).

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