The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.
Gold, B., Roberts, J., Ling, Y., Lopez Quezada, L., Glasheen, J., Ballinger, E., Somersan-Karakaya, S., Warrier, T., Nathan, C. Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria. J. Vis. Exp. (118), e54690, doi:10.3791/54690 (2016).