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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
 

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Article DOI: 10.3791/54615-v • 08:17 min • October 30th, 2016
October 30th, 2016 •
*1,2, *3,4, 1,2, 1,2
1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo
* These authors contributed equally

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요약
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A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.

Tags

Flow Cytometry-based Assay NK Cell Functions Monitoring Viral Infections Malignant Diseases Scientific Research Clinical Evaluations Pediatrics Immunodeficient Patients NK Cell Subsets NK Cell Isolation Cocktail Solution EDTA Peripheral Whole Blood Tube Rotator Magnetic Separator Sterile Conditions Complete Medium
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