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Medição de Inibição da Neuraminidase Influenza títulos de anticorpos por Enzyme-linked Lectina Ensaio
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Medição de Inibição da Neuraminidase Influenza títulos de anticorpos por Enzyme-linked Lectina Ensaio
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September 06, 2016
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We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.
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Gao, J., Couzens, L., Eichelberger, M. C. Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay. J. Vis. Exp. (115), e54573, doi:10.3791/54573 (2016).
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