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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
 

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

Article DOI: 10.3791/54573-v • 08:11 min • September 6th, 2016
September 6th, 2016 •
1, 1, 1
1Division of Viral Products, CBER, Food and Drug Administration

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We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

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Enzyme-linked Lectin Assay Neuraminidase Inhibition Antibody Titers Influenza Virus Surface Vaccine Field Antibody Response Large Numbers Of Serum Samples Reassortant Influenza Viruses H6 Subtype Biosafety Level 2 Enhanced Procedures H6 Reassortant Viruses Chemically Inactivated 96-well Plate Sample Diluent Virus Dilutions
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