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“Phagosome Closure Assay” to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
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“Phagosome Closure Assay” to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
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August 26, 2016
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Summary
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We describe an experimental setup to visualize with unprecedented high resolution phagosome formation and closure in three dimensions in living macrophages, using total internal reflection fluorescence microscopy. It allows monitoring of the base of the phagocytic cup, the extending pseudopods, as well as the precise site of phagosome scission.
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Marie-Anaïs, F., Mazzolini, J., Bourdoncle, P., Niedergang, F. "Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM). J. Vis. Exp. (114), e54470, doi:10.3791/54470 (2016).
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