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準備とシリアルフェムト秒結晶学のための脂質立方相におけるタンパク質微結晶の配達
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생화학
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JoVE 신문 생화학
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
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準備とシリアルフェムト秒結晶学のための脂質立方相におけるタンパク質微結晶の配達

11,043 Views

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:
10.3791/54463-v

•

09:09 min
September 20, 2016

•

9 Views
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1The Bridge Institute,University of Southern California, 2Department of Chemistry,University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Tags

Protein MicrocrystalsLipidic Cubic PhaseSerial Femtosecond CrystallographyMembrane Protein StructureProtein ReconstitutionSample PreparationSyringe HandlingPrecipitant SolutionProtein-laden LCPTeflon SpherulesSample Sealing

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Ishchenko, A., Cherezov, V., Liu, W. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. J. Vis. Exp. (115), e54463, doi:10.3791/54463 (2016).

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