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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:
10.3791/54463-v

•

09:09 min
September 20, 2016

•

9 Views
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1The Bridge Institute,University of Southern California, 2Department of Chemistry,University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Tags

Protein MicrocrystalsLipidic Cubic PhaseSerial Femtosecond CrystallographyMembrane Protein StructureProtein ReconstitutionSample PreparationSyringe HandlingPrecipitant SolutionProtein-laden LCPTeflon SpherulesSample Sealing

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Ishchenko, A., Cherezov, V., Liu, W. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. J. Vis. Exp. (115), e54463, doi:10.3791/54463 (2016).

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