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生物化学
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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

11,150 Views

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:
10.3791/54463-v

•

09:09 min
September 20, 2016

•

9 Views
, ,
1The Bridge Institute,University of Southern California, 2Department of Chemistry,University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Summary

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We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

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Ishchenko, A., Cherezov, V., Liu, W. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. J. Vis. Exp. (115), e54463, doi:10.3791/54463 (2016).

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