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High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
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High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

10,799 Views

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10:25 min

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August 13, 2016

DOI:

10.3791/54265-v

DOI:
10.3791/54265-v

•

10:25 min
August 13, 2016

•

5 Views
, , ,
1Department of Biological Sciences,University of the Sciences in Philadelphia

Summary

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Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

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Braun, A., Caesar, N. M., Dang, K., Myers, K. A. High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells. J. Vis. Exp. (114), e54265, doi:10.3791/54265 (2016).

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